qPCR Talks -- eConferences streaming server

We are presenting the qPCR & NGS eConferences series 2010 - 2015 via video stream!

eConferences

Amplify your knowledge in qPCR, dPCR and NGS!

This streaming portal is dedicated to scientists from the community of qPCR, digital PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx). You’ll find here all the records from around 280 presentations held at qPCR & NGS and MG Events in the past years – qPCR 2010 in Vienna, qPCR 2011, qPCR & NGS 2013 and qPCR & NGS 2015 in Freising-Weihenstephan, and MicroGenomics 2014 in Paris.

We provide the presentations via movie streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device, including Android or iOS.



   

qPCR 2009 - Proceedings, Talks and Posters are free for download !

qPCR 2007  - Symposium Presentations

All scientific contributions are published in the qPCR 2007 Symposium Proceedings

Download the talks and poster presentations from previous qPCR Symposia


RefGenes - a unique tool to find suitable reference genes

Interpretation requires context - making sense out of gene lists and networks
RefGenes was presented at the 2011 qPCR Conference, Weihenstephan, Germany

RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization.
Hruz T, Wyss M, Docquier M, Pfaffl MW, Masanetz S, Borghi L, Verbrugge P, Kalaydjieva L, Bleuler S, Laule O, Descombes P, Gruissem W and P Zimmermann
BMC Genomics 2011, 12: 156

General problem
The choice of suitable reference genes is absolutely crucial in RT-qPCR gene expression analysis. Often, genes from commercial panels don't work well for one's own biological context. Ideally, the expression of reference genes should remain unchanged across samples within the context under study.

Solution
RefGenes is an online app from Genevestigator that allows users to search for genes that are most stable across a chosen set of samples based on microarray data. This set of samples can be chosen according to experimental conditions or tissue types. For example, if you are performing a RT-qPCR experiment on mouse liver samples, you can use RefGenes to identify the set of genes that are most stable across all microarrays done on mouse liver in Genevestigator. This method offers two major improvements over existing methods because a) it does not narrow down from a small set of genes (e.g. commercial housekeeping gene panels), but looks for novel candidates from a genome-wide set of genes b) it is based on condition-specific stability. The below schema shows how RefGenes can be used in combination with existing approaches to yield valuable reference genes for specific experimental conditions.


  • Some Talks from the qPCR Meeting in London 2003, organized by Stephen Bustin:
  • Talks by  J.M. Gallup, Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250, USA.:
  • qPCR Talk  by Renee Horner - Queen of qPCR   download PDF
  • Determination of real-time PCR efficiency  - An overview
    held at the qPCR 2004 Event     download PDF