qPCR 2005 - Symposium talks

qPCR 2005 - Symposium Talks

qPCR 2005 Symposium Proceedings ( ISBN 3000166874 )

Session	Speaker	Title
Keynote speaker	Russell Higuchi "Pioneer in real-time PCR" Associate Director of the Human Genetics Department, Roche Molecular Systems, Almeda, CA, USA	Real-time PCR, a personal perspective.
Pre-analytical steps	Stephen A Bustin Professor of Molecular Science Institute of Cell and Molecular Science Queen Mary's School of Medicine and Dentistry, University of London	mRNA quantification from archival cancer samples. Normalisation of mRNA levels against total DNA content.
Bioinformatics	Mikael Kubista Professor of Biotechnology, MultiD Analyses AB, Göteborg, Sweden	Classification of real-time PCR data.
New Applications	Neven Zoric Coordinator of the TATAA Biocenter Göteborg, Sweden	The best of both worlds - New dyes for qPCR for use in combination with probes.
Pre-analytical steps	Michael W. Pfaffl Reader in Physiology Center of Life Science, Weihenstephan Technical University of Munich, Germany	Influence of RNA matrix effect on qRT-PCR results - an overview.
Array Verification	Elisa Wurmbach Assistant Professor Mount Sinai School of Medicine Department of Hematology/Oncology New York, NY, USA	Correlation of microarray and quantitative real-time RT-PCR results.
Optimization	Greg Shipley Director, Quantitative Genomics Core Laboratory, The University of Texas Health Science Centre- Houston, USA	Design and optimization of Taqman and SYBR Green I real-time qPCR assays. Shipley-2-qPCR-2005.pdf
Standardization	Pamela Scott Adams Director, Molecular Biology Core Facility, Trudeau Institute, Saranac Lake, NY, USA	A Comparison of Real-Time RT-PCR Technique, Chemistries and Instrumentation in Laboratories Utilizing the Same Assay.
Normalization	Jo Vandesompele Center for Medical Genetics Ghent Ghent University Hospital, Ghent, Belgium	Normalization of gene expression: state of the art and preview on a new strategy using expressed Alu repeats.
BioInformatics	Jan Hellemans Center for Medical Genetics Ghent Ghent University Hospital, Ghent, Belgium	qBase: relative quantification software for management and automated analysis.
New Applications	Bernadett Papp Gene Expression Unit EMBL, Heidelberg, Germany	ChIP experiments from Drosophila tissues analyzed by qRT-PCR.
Optimization	Anders Stahlberg Department of Chemistry and Biosciences Chalmers University of Technology Göteborg, Sweden	Single Cell Transcriptome.
GMO	Ron Jenkins USDA/GIPSA USA	The USDA/GIPSA Proficiency Program: A Summary of Participants Capabilities for Detecting and Quantifying Transgenic Events in Corn and Soybeans.
Keynote speaker GMO	Philipp Hübner Kantonales Laboratorium Basel-Stadt, Abteilungsleiter Lebensmittel, Basel, Switzerland	Uncertainties and certainties in GMO analytics using qPCR.
GMO	Isabel Taverniers Ministry of the Flemish Community,Centre for Agricultural Research – CLO,Dept. for Plant Genetics and Breeding – DvP, Belgium	Cloned plasmid DNA molecules as a tool for GMO analysis.
GMO	Francisco Moreano Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Infektologie / Molekularbiologie, Germany	Application of synthetic DNA-standards for the quantitative screening of different genetically modified rapeseed lines via real-time PCR.
New Applications	Kristina Lind Department of Chemistry and Biosciences Chalmers University of Technology Göteborg, Sweden	Real-time immuno-PCR for the quantification of proteins.
Bioinformatics	Tzachi Bar & Ales Tichopad LabonNet, Silberhornstrasse 3, Kirchheim bei München, Germany	Efficiency estimation from single amplification curve – methods and applications.
Standardization	Bob Rutledge Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre Sainte-Foy, Quebec, Canada	Putting the "quantity " into quantitative PCR: A simplified approach to the establishment and application of quantitative scale.
Bioinformatics	Bob Rutledge	Amplification efficiency dynamics and its implications: Developing a kinetic-based approach for quantitative analysis.
Standardization	Morten T. Andersen Bio-Molecular Innovation, LGC Teddington, Middlesex, UK	The Data Comparability Challenge - Standards and Best Practices.
New Applications	Jim Huggett Centre for Infectious Diseases & International Health, University College London, UK	Infectious disease diagnostic research in Africa; the role of real time PCR.
New Applications	Arthur Reis Brandeis University, Boston, MA, USA	Forensic and Single-Molecule Assays of Mitochondrial DNA Using LATE-PCR.

	Speaker
New Applications	Michael Boutros Boveri-Group Signaling and Functional Genomics, DKFZ, Heidelberg, Germany sponsored by Roche Applied Science	Rapid Development of RT-PCR Assays for RNAi Experiments Using Pre-designed LNA-probe Libraries.
Optimization	Raimund Kinne Experimentelle Rheumatologie, Klinikum der Friedrich-Schiller-Universität Jena , Germany sponsored by Eppendorf	Comparison of MMP gene expression analysis by capillary and "realplex" real-time PCR.
Food Hygiene	Martina Fricker Dep. of Bioscience, Technical University of Munich, Freising, Germany sponsored by Cepheid	Detection of Food Pathogens using the Smart Cycler II.
Pre-analytical steps	James Willey Medical University of Ohio, TOLEDO, OH 43614, USA	Use of standardized mixtures of internal standards in RT-PCR to generate validated biomarkers and to develop standardized transcript abundance reference databases.

	Speaker
Standardization	Oliver Geulen Roche Applied Science, Mannheim, Germany	Accurate Gene Expression Analysis with High Flexibility: Concepts and Developments.
Optimization	Cynthia Potter Eppendorf UK	Validation of fast PCR protocols with the Eppendorf Mastercycler ep realplex.
Pre-analytical steps	Guido Hennig Bayer HealthCare AG Diagnostics Research Germany Leverkusen	Nucleic acid isolation for diagnostic testing using Bayer´s magnetic particles.
Optimization	Olfert Landt TIB MOLBIOL Syntheselabor GmbH Eresburgstraße, Berlin, Germany	Finding the needle in the haystack - LNA bases enhance SNP detection dramatically.
GMO	Dörte Wulff Research and Development Eurofins Genescan / GeneScan Analytics GmbH, Freiburg, Germany	Accurate GMO quantification in food samples.
New Applications	Andreas Missel Associate Director R&D, QIAGEN GmbH, Hilden	Going MULTI – how to easily achieve high multiplexing in real-time PCR.
New Applications	Hilary K. Srere Bio-Rad Laboratories Europe, Munich, Germany	The Ups and Downs of Gene Regulation: Validating siRNA Gene Expression Disruption with RT-qPCR.
Pre-analytical steps	L. Scott Basehore Sr. Research Associate, Stratagene Research & Development Department	Nucleic Acid Stabilization in Cultured Cell and Tissue Lysates for QPCR Gene Expression Analysis.
New Applications	Kyle Hooper Promega Corporation, Woods Hollow Road, Madison, WI, USA	Plexor™ Real-Time Quantitative PCR Systems: Multiplexed assays made easy.
Optimization	Clemence Beslin product manager, Eurogentec, Belgium	qPCR pitfalls - primer and probe design / fluorophore quencher combinations.
Optimization	Thomas Kaiser Corbett Research, Sydney, Australia	The fitness of a football team: High Resolution Melts for the determination of genotypes.
New-Applications	Mark Andersen Invitrogen, USA	Two-color multiplex assay for the identification of Orthopoxes viruses with Real-Time LUX PCR.
Transcriptomics	Simone Guenther Applied Biosystems, Applera Deutschland GmbH, Darmstadt, Germany	microRNA expression profiles from real-time PCR classify ES and differentiated cells.
Bioinformatics	Ben Sowers Research Associate, Biosearch Technologies	From Sequences to Synthesis: Optimal Amplification through Careful Oligonucleotide Selection.
Pre-analytical steps	Marc Valer Agilent Technologies, Waldbronn, Germany	Standardization of RNA Quality Assessment using the RNA Integrity Number (RIN) and the 2100 bioanalyzer.
Standardization	Andreas Eckelt Cepheid SA, Germany	Assay standardisation using universal internal controls and lyophilized reagent beads.
All scientific contributions are published in the qPCR 2005 Symposium Proceedings ISBN 3000166874