HRM - High Resoltution Melt (3)
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HRM page (2)
HRM Dyes

NEW HRM application papers:

Determination of KCNQ1OT1 and H19 methylation levels in BWS and SRS patients using methylation-sensitive high-resolution melting analysis.
Alders M, Bliek J, vd Lip K, vd Bogaard R, Mannens M.
Eur J Hum Genet. 2009 17(4): 467-473.

High quality assessment of DNA methylation in archival tissues from colorectal cancer patients using quantitative high-resolution melting analysis.
Balic M, Pichler M, Strutz J, Heitzer E, Ausch C, Samonigg H, Cote RJ, Dandachi N.
J Mol Diagn. 2009 (2):102-108.

Complete discrimination of six individuals based on high-resolution melting of hypervariable regions I and II of the mitochondrial genome.
Olof Gidlöf, Sofia Burvall, Lars Edvinsson, Maria Montelius, Marie Allen, Magnus Molin
BioTechniques, Vol. 47, No. 2, August 2009, pp. 671–678

Identifying sequence variants in the human mitochondrial genome using high-resolution melt (HRM) profiling.
Dobrowolski SF, Gray J, Miller T, Sears M.
Hum Mutat. 2009 (6): 891-898.

Quantitative evaluation of DNA methylation by optimization of a differential-high resolution melt analysis protocol.
Malentacchi F, Forni G, Vinci S, Orlando C.
Nucleic Acids Res. 2009 (12): e86

Mutation scanning using high-resolution melting.
Taylor CF.
Biochem Soc Trans. 2009 37(Pt 2): 433-437. Review.

High-resolution melting analysis (HRMA): more than just sequence variant screening.
Vossen RH, Aten E, Roos A, den Dunnen JT.
Hum Mutat. 2009 30(6): 860-866.

Methylation-sensitive high-resolution.
Wojdacz TK, Dobrovic A, Hansen LL.
melting. Nat Protoc. 2008; 3(12): 1903-1908.

Primer design versus PCR bias in methylation independent PCR amplifications.
Wojdacz TK, Borgbo T, Hansen LL.
Epigenetics. 2009 (4): 231-234

Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons.
Gundry CN, Dobrowolski SF, Martin YR, Robbins TC, Nay LM, Boyd N, Coyne T, Wall MD, Wittwer CT, Teng DH.
Nucleic Acids Res. 2008 36(10): 3401-3408.

High-resolution melting analysis (HRMA):a highly sensitive inexpensive genotyping alternative for population studies.
Molecular Ecology Resources (2009) 1-4

Multiplex Amplicon Genotyping by High-Resolution Melting.
Michael T.Seipp, Jacob D. Durtschi, Karl V. Voelkerding, and Carl T. Wittwer
Communication in Journal of Biomolecular Techniques (2009) 20: 160–164

Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner.
van der Stoep N, van Paridon CD, Janssens T, Krenkova P, Stambergova A, Macek M, Matthijs G, Bakker E.
Hum Mutat. 2009 30(6): 899-909.

Quantitative PCR high-resolution melting (qPCR-HRM) curve analysis, a new approach to simultaneously screen point mutations and large rearrangements: application to MLH1 germline mutations in Lynch syndrome.
Rouleau E, Lefol C, Bourdon V, Coulet F, Noguchi T, Soubrier F, Bièche I,
Olschwang S, Sobol H, Lidereau R.
Hum Mutat. 2009

Quadruplex Genotyping of F5 F5, F2 F2, and MTHFR Variants in a Single Closed Tube by High-Resolution Amplicon Melting.
Michael T. Seipp, David Pattison, Jacob D. Durtschi, Mohamed Jama, Karl V. Voelkerding, and Carl T. Wittwer
Clinical Chemistry 54:1 (2008)

High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus.
Alex J. Stephens, John Inman-Bamber, Philip M. Giffard, and Flavia Huygens
Clinical Chemistry 54:2 432–436 (2008)

Detection of alpha-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis.
Sakorn Pornprasert, Arunee Phusua, Sudjai Suanta, Rattika Saetung, Torpong Sanguansermsri
Eur J of Haematol. 2008.

Sensitive Melting Analysis after Real-Time Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection.
Lasse S. Kristensen, Thomas Mikeska, Michael Krypuy, Alexander Dobrovic
Nucleic Acids Research 2008, March, p1-13

A new approach to primer design for the control of PCR bias in methylation studies.
Wojdacz TK, Hansen LL, Dobrovic A.
BMC Res Notes. 2008 Jul 28;1:54.

High-resolution melt curve analysis: Initial screening for mutations in BCR-ABL kinase domain.
Poláková KM, Lopotová T, Klamová H, Moravcová J.
Leuk Res. 2008 Feb 26

BRCA1 promoter methylation in peripheral blood DNA of mutation negative familial breast cancer patients with a BRCA1 tumour phenotype.
Cameron Snell, Michael Krypuy, Ee Ming Wong, Maurice B Loughrey and Alexander Dobrovic
Breast Cancer Research 2008, 10:R12

Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System.
Grievink H, Stowell KM.
Anal Biochem. 2008