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The Reference in qPCR & dPCR - Academic & Industrial Information Platform

Since 20 years, the Gene Quantification platform
describes and summarises all technical
aspects involved in quantitative gene expression analysis using real-time qPCR & digital-PCR & Next Generation Sequencing.  It presents a lot of new and innovative qPCR & dPCR applications, chemistries, methods, algorithms, MIQE and QC strategies, cyclers, kits, dyes, analysis methods, events, and services involved.  Browse our page directory commercial and academic institutions can present their Gene Quantification, qPCR and dPCR tools right here on our qPCR platform.
Amplify your knowledge in qPCR, dPCR and NGS -- Follow our streaming server with 420 talks --      

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MIQE & iBook / PDF NEW

EV & Exosomes & EV RNA / Liquid Biopsy / Biomarker Discovery Transcriptional Biomarkers / qPCR Evolution
Books & eBooks ... NEW
BDQ / Protocols / MethodsTranslation / ASIA portal / GQ Page Directory / qPCR Platform
eConferences & Webinars

Free access to 420 recorded talks Gene Quantification Events 2010-2019 / Talks / Webinars / Podcasts 
Quantification Strategy
Quantification strategies in RT-qPCR:  absolute Quantification & relative Quantification

RT-qPCR normalisation strategies & microRNA / Genorm / BestKeeper / RefGenes ... UPDATE
qPCR & Optimisation
real-time PCR / interesting reviews / MIQE & Apps & iBook ... NEW / Primer-Recources & Algorithms
Bioinformatics & Statistics
Big Data in Biology ... NEW / qPCR data analysis / Regulatory Networks data clustering / Integrated Analysis
Software Downloads

REST (main page) / REST-2009 / RDML / RefGenes / GenEx 7.0 ... NEW VERSION ... FREE DOWNLOAD
RNAi -- RNA interference

siRNA / saRNA / microRNA & microRNA normalisation / mirtron  ... NEW / qPCR statistics
qPCR dPCR NGS Events
Next Event => #GQ2023 / qPCR dPCR NGS 2023 / eConferences / previous qPCR Events
digital PCR  & qPCR Array
digital PCR ... UPDATE / MIQE & dPCR / qPCR array / normalisation and analysis software
Cyclers  & Robotics
real-time PCR hardware:  overview of qPCR cyclers  / qPCR robotics / page statistics
reverse transcription / mRNA transcript analysis ... UPDATE / RNA QC & RNA ... UPDATE / DNA integrity

Dyes  & HRM dyes

detection dyes, probes, and chemistries in real-time PCR / HRM dyes / Chips & Lab-on-Chip
Liquid Biopsy & Biomarkers
Exosomes / EV & Exosomal RNA ... UPDATE / CNA / microRNA / Transcriptional Biomarkers
Molecular Physiology
CNA / HRM / CNV / microRNA / siRNA / saRNA / RNAi / digital PCR ... UPDATE / single-cell qPCR
qPCR Efficiency
determination of real-time qPCR efficiency by various methods ... new efficiency papers !
qPCR & dPCR applications

Exosomes / CNA (circulating nucleic acids) / digital PCR ... UPDATE / single-cell handling / single-cell qPCR
GQ info portal

microRNA  / CNV / microRNA transfer / HRM / RNA interference / siRNA / saRNA / Liquid Biopsy

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is sponsored by
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qPCR platform participating companies:
qPCR platform participating companies in alphabetical order

logo Bio-Rad Bio-Rad Laboratories - A complete solution.  A focused approach.
BioMolecular Systems - Meet Mic. Fast. Accurate. Compact.

Eurogentec logo
Eurogentec - Your one-stop-shop Real-Time PCR supplier

logo Analytik
Analytik Jena offers a wide produt variety of thermal cyclers as well as real-time thermal cyclers.
Biozym button
Biozym - Science is our business
button NEB New England BioLabs -- Luna® qPCR and RT-qPCR Reagents for rapid, sensitive and precise detection of RNA, DNA and cDNA targets
logo GNA Biosolutions
GNA Biosolutions - Simplified molecular testing in minutes
logo Bioline
Bioline – The PCR Company
button Promega real-time PCR Promega - Be successful in your qPCR experiments with our robust amplification master mixes

logo xxpresspcr
BJS Biotechnologies - xxpress® the world’s fastest qPCR system
button Stilla Technologies
Stilla Technologies - Naica System Workflow - a fast and simple workflow Life Technologies - Advanced gene expression services

TIB-Molbiol - Our business is DNA.
Biosearch Technologies - Advancing Nucleic Acid Technologies
Quanta BioSciences
QuantaBio is dedicated to perfecting qPCR reagents that deliver superior performance you can rely on.
logo nexttec
nexttec Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

logo BioSistemika BioSistemika - Software development partners for life sciences - Helping deliver your ideas! PrimerDesign - Driving technology, Empowering researchers
logo LTF Labortechnik LTF Labortechnik - Results at a glance !

Your want to join the qPCR or eConferences platform!  Contact us via

Cooperating Institutions & Laboratories:

TATAA Biocenter is a research provider that offers training and commissioned services within molecular diagnostics and gene expression analysis using real-time PCR and other molecular techniques to quantify nucleic acids. Our competence is based on knowledge and experience accumulated through years of research at leading European Universities. Our offer comprises the entire field of real-time PCR services, including commissioned research, hands-on training, and custom design of real-time PCR assays.
Further activities:  
1)  Open and tailor-made hands-on training courses;    2)  Commissioned research and development;   3)  Custom design of qPCR assays and assay validation;   4)  Product development          Express your genius with qPCR
logo MultiD -- Discuss GenEx and qPCR
Welcome to qPCRforum - Forum for discussion about GenEx and qPCR (quantitative real-time PCR)

GenEx software -
GenEx offers advanced methods to analyze real-time qPCR data with simple clicks of the mouse. The methods are suitable to select and validate reference genes, classify samples, group genes, monitor time dependent processes and much more.

Stephen Bustin Stephen Bustin is Professor of Molecular Medicine at Anglia Ruskin University  at the Postgraduate Medical Institute.

Department of Gene Expression
Mikael Kubista´s
lab is at the Institute of Molecular Genetics AS CR ( and his group is developing real-time PCR assays for different applications such as routine clinical diagnostic and methods for high through put measurements.    TATAA Biocenter Prague

GeneCore is the in-house genomics service center at EMBL for DNA sequencing, next-generation-sequecing, S.A.G.E., microarrays and real-time qPCR. Services are provided on non-commercial basis for the EMBL community and academic institutions in the context of scientific collaborations within the EMBL member states. Tight links to industry provide state-of-the-art equipment. The experiments and their results feed directly back into improvements of instruments and technologies, spawning the next generation of scientific questions and technologies.

Biogazelle  is the quantitative PCR data-analysis company, founded in 2007 as a Ghent University spin-off by Prof. Jo Vandesompele and Dr. Jan Hellemans. Its founders have more than 15 years of experience in real-time PCR experiment design, assay development and data-analysis. They wrote one of the most influential papers on normalization of gene expression and on data-analysis (together cited more than 10,000 times). Biogazelle's flagship product qbase+ is the most powerful, flexible, and user-friendly real-time PCR data-analysis software based on the proven geNorm and qBase technology, enhanced with proprietary algorithms and time-saving features. qBASE+ is truly accelerating your research.

geNorm VBA applet for Microsoft Excel determines the most stable reference genes from a set of tested genes and calculates a gene expression normalization factor based on the geometric mean of a user-defined number of reference genes.
qBase is a collection of macros for Microsoft Excel
for the management and automated analysis of real-time quantitative PCR data. The program employs a delta-Ct relative quantification model with PCR efficiency correction and multiple reference gene normalization.

qPCR Platform Media Partner:


Technology Networks were established around 15 years ago with the aim of providing informative websites for life science professionals.
From our UK headquarters near Sudbury, in Suffolk, we run, update and provide an outstanding web-based information solution for those working within the Life Science and Drug Discovery community. Since its humble beginnings running just one website based on Combinatorial Chemistry, Technology Networks has expanded its portfolio to include over 30 communities:
e.g.  qPCR  Next Gen Sequencing  AgriGenomics  Molecular & Clinical Diagnostics  Genomics  Genotyping & Gene Expression 

BIOCOM AG - Information and Communication for the Biotechnology is edited by Michael W. Pfaffl
The Gene Quantification web pages are educational and academic sites with the only purpose of facilitating access to biology-related information over the internet.
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